Fluorescence Lifetime Techniques in Medical Applications
نویسندگان
چکیده
منابع مشابه
Handheld multispectral fluorescence lifetime imaging system for in vivo applications.
There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed clinically compatible systems. We present a handheld probe design consisting of a small maneuverable box fitted with a rigid endoscope, capable of continuous lifetime imaging at multi...
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During the last two decades, the use of fluorescence in natural sciences has propelled a number of research fields, ranging from biological sciences and applications in the clinical sector (FISH for genetic testing, advanced sequencing technology) to environmental monitoring. In addition, the resulting technological developments have become important in several fields of materials sciences, for...
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A novel high-speed fluorescence lifetime imaging (FLIM) analysis method based on artificial neural networks (ANN) has been proposed. In terms of image generation, the proposed ANN-FLIM method does not require iterative searching procedures or initial conditions, and it can generate lifetime images at least 180-fold faster than conventional least squares curve-fitting software tools. The advanta...
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Fluorescence Lifetime Correlation Spectroscopy (FLCS) is a variant of fluorescence correlation spectroscopy (FCS), which uses differences in fluorescence intensity decays to separate contributions of different fluorophore populations to FCS signal. Besides which, FLCS is a powerful tool to improve quality of FCS data by removing noise and distortion caused by scattered excitation light, detecto...
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Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution lifetime images of thin tissue sections. This chapter concerns the in vivo time domain imaging of fluorescent contrast agents embedded in de...
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ژورنال
عنوان ژورنال: Annals of Biomedical Engineering
سال: 2012
ISSN: 0090-6964,1573-9686
DOI: 10.1007/s10439-011-0495-y